Genomics and Proteomics Core Laboratories
The Biomedical Research Support Facility is a group of specialty core facilities developed to enhance the research environment at the University of Pittsburgh and to optimize the productivity and competitiveness of researchers. The cores provide an infrastructure that allows research to remain competitive and take advantage of remarkable shifts in our fundamental understanding and applications of cell and animal model systems. A brief description of the cores follows:
- Bioinformatics Analysis Core
The Bioinformatics Analysis Core (BAC) of the Genomics and Proteomics Core Laboratories provides data analysis services for a broad range of data types. We are available to consult and participate in basic and clinical study design, including patient accrual algorithms and multivariable case/control selection.
- DNA/RNA Extraction
The specimen processing and banking laboratories have extensive RNA/DNA extraction experience for dozens of tissue types and species. Extraction can be performed in standard or high throughput formats. Whole Genome Amplification is also available. Secure specimen banking facilities are available.
- DNA Analysis
We offer genetic and genomic analyses for a variety of applications. Sequencing is available in standard and high throughput formats. The choice of Taqman, Affymetrix or Illumina based SNP assay platforms is based on project goals and throughput needs.
- Gene Expression Analysis
Our MicroArray and Taqman Real Time PCR offerings allow investigators to obtain gene expression data from a flexible array of formats. Full service is available for all offerings including Affymetrix and Illumina gene expression Array products.
- Protein Profiling
Protein profiling and separations technologies are often customized based on the needs of each project. Standardized processes include traditional and Cy-dye (DiGE) based 2 dimensional (2D) gel electrophoresis (PAGE). Nano flow Mudpit analyses are available in MDLC MALDI and ion trap methods.
- Protein ID
Protein identification and characterization processes include Peptide Mass Fingerprinting (PMF), de novo sequencing, Post Translational Modification (PTM) analysis and many other chemical and mass spectrometry techniques.
Peptide Systhesis Core
This core provides comprehensive services for synthesis, purification, and characterization of synthetic peptides that are verified by mass spectrometry. Facility personnel are available for consultation with investigators regarding the design of synthesis peptides, estimates of yield, and considerations of purity requirements. Peptides can be produced at standard scales of ~0.025 mM (10-20mg), 0.1 mM (50-100mg), 0.2 mM (100-200mg), and 0.5 mM (300-500mg), with actual yields dependent on peptide length and content. Peptides may also be prepared with specialized modification, such as acetylation, biotinylation, phosphorylation, cyclization, or fluorescent dyes. The facility has the capacity to produce certified peptides for use in human clinical trials with appropriate production documentation for submission to the FDA and other regulatory agencies.
Center for Biological Imaging
The Center for Biomedical Imaging is located adjacent to the CVR in the Biomedical Science Tower 2 and provides centralized imaging services including light fluorescent microscopy, confocal laser scanning, electron microscopy, advanced computer aided morphometry, and image analysis.